Proceedings of the International scientific and practical conference “Multidisciplinary approaches in science, technology and culture” (October 21-23, 2025) / Publisher website: www.naukainfo.com. – Berlin, Germany, 2025. - 121 p.

27 weak intermolecular interactions and partial cell death may occur, leading to the release of water-soluble components into the aqueous phase. It was found that when the yeast suspension was incubated at 6 °C for 24 h, 0.6 g/L of dry matter was released into the aqueous medium. After 96 h of incubation, the concentration of released substances doubled, while further prolongation up to 8 days did not significantly increase the yield compared with the 96-hour incubation. At the next stage, we analyzed the spectral composition of the substances present in the water-soluble fractions obtained after pre-adaptation at 6 °C for 24 and 96 h, followed by autolysis for 4 days and autoclaving at 121 °C, 1.5 atm for 10 min. It was observed that after incubation at 6 °C for 24 h, a slight absorption was detected in the 200–205 nm region, decreasing linearly up to 240 nm, with minor absorption at 260–270 nm typical of proteins and nucleic acids (Fig. 1). Figure 1: Typical absorption spectra of water-soluble yeast components obtained after pre-adaptation, autolysis, and autoclaving steps.