Proceedings of the International scientific and practical conference “Multidisciplinary approaches in science, technology and culture” (October 21-23, 2025) / Publisher website: www.naukainfo.com. – Berlin, Germany, 2025. - 121 p.

26 production [7]. Accordingly, a pilot-scale bioreactor was designed with modules for temperature regulation and mixing intensity control. Methods. For feeding and draining the suspension, dosing pumps or solenoid valves were used – a methodological approach described as a “hybrid solution,” most suitable for pilot-scale applications [8]. Temperature regulation of the medium was provided by a thermostatic water jacket and simple PID controllers combined with periodic mixing. Such an approach reduces process variability and enhances reproducibility, which is particularly important when scaling up from pilot conditions [9]. The cleaning procedure was performed using the Clean-in-Place (CIP) protocol [10]. Process parameter data were recorded in Excel spreadsheets, ensuring control over key parameters for each batch. This approach provides a foundation for future implementation of more advanced MES/SCADA systems and facilitates process scalability [11]. Baker’s yeast S. cerevisiae was used in this work. A 33% yeast cell suspension was prepared in distilled water (calculated on a dry weight basis). Various sample variants were then prepared for subsequent processing. Results and Discussion. To modify the structural-functional organization of yeast cells, pre-adaptation of the culture to a reduced temperature (6 °C) was conducted. It is known that transferring cultures to non-optimal (stressful) conditions leads to a reduction in overall metabolic activity and induces the synthesis of stress-related proteins, resulting in structural and functional reorganization of cellular components, including membranes. When the temperature decreases below 10 °C, enzyme activity inside the cell drops by 80–90% of the baseline value. Under such conditions, the destruction of