Proceedings of the International scientific and practical conference “Multidisciplinary approaches in science, technology and culture” (October 21-23, 2025) / Publisher website: www.naukainfo.com. – Berlin, Germany, 2025. - 121 p.

25 provides an almost complete release of all intracellular water-soluble compounds [1]. The efficiency of obtaining WSCs through such a multistage technology requires strict adherence to operational parameters, which can only be ensured through process automation – the main objective of this study. Keywords : water-soluble yeast components, autolysis, pre-adaptation, autoclaving, automation, Saccharomyces cerevisiae Introduction. The development and improvement of technologies for obtaining biologically active substances from Saccharomyces cerevisiae yeast remain an urgent scientific and industrial task. This is explained by several factors: (1) a large amount of yeast biomass generated during beer and other food production processes remains unused [2]; (2) yeast biomass contains up to 40% protein, B-group vitamins, a vitamin D precursor (ergosterol), trace elements, and other valuable substances, serving as a raw material for feed, food, and pharmaceutical industries [3]. However, this enormous potential of yeast biomass remains underutilized due to the lack of economically feasible technologies for deep biomass processing. Currently, only yeast autolysate production methods are commonly applied [4]. Despite various modifications of the autolysis process, these methods typically provide a relatively low yield of the target product, leaving part of the biomass unprocessed and contributing to organic waste pollution of the environment [5]. We suggest that the development of relatively low-cost technological systems for yeast biomass processing – equipped with automated control modules, ensuring modularity and operational flexibility (the ability to vary process conditions over a wide range) – will significantly contribute to the implementation of new technologies for obtaining valuable biologically active substances not only from yeasts but also from other biological materials [6]. We proceeded from the assumption that the automation of biotechnological processes is a key factor in improving the reproducibility, safety, and efficiency of