Proceedings of the International scientific and practical conference ―Science, technology and cultural innovations‖ (October 7-9, 2025) / Publisher website: www.naukainfo.com. – Vienna, Austria, 2025. - 138 p.

80 This is achieved by the use of myo-inositol, spironolactone, and vitamin D3, specifically: 2 g of myo-inositol per day, 100 mg of spironolactone per day, and 2000 IU of vitamin D3 per day. This treatment should continue for 6 months. We recommend undergoing such preventive treatment from October to April, at least for several years. It should be emphasised the importance of healthy eating, combating harmful habits, and physical activity [5]. Biologically active supplements containing Myo-Inositol are an alternative treatment for teenage girls with PCOS. Given the significant potential of Myo- Inositol to influence metabolic parameters and its minimal side effects, it can be recommended for use in PCOS [6]. Today, dietary supplements containing Myo- Inositol are recommended for use by International Evidence-based Guideline for the assessment and management of polycystic ovary syndrome, ESHRE, 2023. Concerning antiandrogenic drugs, spironolactone has minimal side effects and is best suited for use in adolescents. It eliminates the clinical manifestations of hyperandrogenism and stabilises the renin-angiotensin-aldosterone system and helping to reduse the symptoms of fluid retention in premenstrual disorders against in adolescents with PCOS [7]. Vitamin D 3 is well known as the main regulator of calcium and phosphorus homeostasis. A significant number of publications indicate its crucial role in regulating of reproductive processes in women. Vitamin D 3 receptors are found in large numbers in the internal reproductive organs, particularly in the ovaries. Importantly, in addition to expressing vitamin D 3 receptors, the ovary is an extrarenal site of vitamin D 3 metabolism. The effect of vitamin D 3 on follicular development and ovarian steroidogenesis has been studied. In addition, vitamin D 3 deficiency is also associated with polycystic ovary syndrome, premature ovarian failure, and ovarian cancer [8]. It is considered appropriate to use a combination of spironolactone, myo-inositol and vitamin D 3 in conjunction with a healthy diet and moderate exercise in adolescent girls with a high risk of polycystic ovary syndrome in order to stop the progression of